Genetic and molecular characterization of Pseudomonas Aeruginosa causing infections in ICU of three cities in Colombia.

Abstract

Pseudomonas aeruginosa, ubiquitous bacteria that can generate complicated infections in hospital patients, increasing morbidity and mortality rates due to increased antimicrobial resistance in clinical use, especially the last therapeutic option as carbapenems for the acquisition of resistance determinants through mainly mobile genetic elements.OBJECTIVE: To characterize the resistance profile and molecular characteristics of P. aeruginosa isolated from adult patients with a diagnosis of infection in three intensive care units in Colombia. METHODS: Patients with P. aeruginosa infections in adults were analyzed UCI. A bacterial isolates were determined profile of susceptibility to 12 antibiotics and resistance genes were amplified β-lactams, quinolones, sulfonamides and genetic platforms like integron class 1 and 2. The genetic relationship by PFGE and MLST. RESULTS: In the study 40 patients of which 23 (57.5%) analyzed belong to an ICU in the city of Pereira. The main sources of isolating microorganisms are 13 blood cultures (32.5%) and urine-11 (27.5%). Resistance profiles SAM-SAM-FOX and FOX-SXT occurred in 6 (15.0%) and 4 (10.0%) isolates respectively. The β-lactamases frequently were blaTEM type in 8 (20.0%), blaSHV seven (17.5%) and blaCTX-M 3 (7.5%), carbapenemases blaVIM blaKPC-2 and type 4 (9.7%) and 3 (7.5%). Isolates presented a polyclonal behavior pulsotypes 28. The KPC-producing isolates 2 and VIM are associated with the ST235 and ST111 respectively. CONCLUSION: those generated in ICUs participating entities infections are highly variable and moderate resistance to carbapenems associated with the presence of KPC-2 and VIM associated with the pandemic clone ST235 and ST111.